Comparison of Viscosities from the Chapman-Enskog and Relaxation Time Methods

A quantitative comparison between the results of shear viscosities from the Chapman-Enskog and relaxation time methods is performed for selected test cases with specified elastic differential cross sections: (i) the non-relativistic, relativistic and ultra-relativistic hard sphere gas with angle and energy independent differential cross section, (ii) the Maxwell gas, (iii) chiral pions and (iv) massive pions. Our quantitative results reveal that the extent of agreement (or disagreement) depends very sensitively on the energy dependence of the differential cross sections employed.Comment: Submitted to Cent. Eur. J.Phy

La structure électromagnétique du deuton

École thématiqu

Multiplex PCR assay for immediate identification of the infecting species in patients with mycobacterial disease

Rapid identification of infecting mycobacterial species enables appropriate medical care decisions to be made. Our aim was to demonstrate the clinical usefulness of the multiplex PCR assay, a test based on PCR, which permits direct identification of 12 mycobacterial species in clinical specimens. A total of 259 specimens from 177 patients who had clinical symptoms of mycobacterial disease but for whom there were difficulties in diagnosis were tested. Specimens were analyzed within 48 h of receipt of the sample. Mycobacteria were identified in 102 specimens; 66 specimens contained nontuberculous mycobacteria, and 36 specimens contained Mycobacterium tuberculosis complex mycobacteria. The PCR assay identified the mycobacterial species in 43 (97.7%) of 44 microscopy- and culture-positive specimens and in 15 (93.8%) of 16 culture-positive, microscopy-negative specimens. It also permitted species identification in infections caused by more than one mycobacterial species. For 56 (96.5%) of the 58 specimens from patients with infections caused by opportunistic mycobacteria, the organisms were identified with the PCR assay. The test was useful also for the identification of fastidious mycobacteria, e.g., M. genavense, and those that cannot be cultured, e.g., M. leprae. After resolution of discrepant results, the sensitivity of the PCR assay was 97.9%, the specificity was 96.9%, the positive predictive value was 95.0%, and the negative predictive value was 98.7%. For culture these values were 60.8, 100, 100, and 81.0%, respectively. Thus, the multiplex PCR assay enables prompt diagnosis when rapid identification of infecting mycobacteria is necessary

Detentiebeleving van strafrechtelijk gedetineerden zonder verblijfsrecht

__Abstract__ The detention experiences of male criminal foreign national prisoners without legal residence receive little attention in penological literature. A qualitative study amongst 30 prisoners with and 16 prisoners without legal residence in the penitentiary institution Tilburg shows that contacts with the social network and the preparation of the reintegration in society are (more) complicated for foreign national prisoners without legal residence. Besides, communication with the staff is more difficult for this group. These factors have negative impact on their detention experiences. The results show that both deprivation and importation theory apply to foreign national prisoners without legal residence. However, importation aspects - especially the lack of legal residence - may substantially and systematically increase the deprivation and result in additional exclusion and isolation mechanisms for this pa

A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples

Diagnostic techniques based on PCR have two major problems: false-positive reactions due to contamination with DNA fragments from previous PCRs (amplicons) and false-negative reactions caused by inhibitors that interfere with the PCR. We have improved our previously reported PCR based on the amplification of a fragment of the Mycobacterium tuberculosis complex-specific insertion element IS6110 with respect to both problems. False-positive reactions caused by amplicon contamination were prevented by the use of uracil-N-glycosylase and dUTP instead of dTTP. We selected a new set of primers outside the region spanned by the formerly used primers to avoid false-positive reactions caused by dTTP-containing amplicons still present in the laboratory. With this new primer set, 16 copies of the IS6110 insertion element, the equivalent of two bacteria, could be amplified 10(10) times in 40 cycles, resulting in a mean efficiency of 77% per cycle. To detect the presence of inhibitors of the Taq polymerase, which may cause false-negative reactions, part of each sample was spiked with M. tuberculosis DNA. The DNA purification method using guanidinium thiocyanate and diatoms effectively removed most or all inhibitors of the PCR. However, this was not suitable for blood samples, for which we developed a proteinase K treatment followed by phenol-chloroform extraction. This method permitted detection of 20 M. tuberculosis bacteria per ml of whole blood. Various laboratory procedures were introduced to reduce failure or inhibition of PCR and avoid DNA cross contamination. We have tested 218 different clinical specimens obtained from patients suspected of having tuberculosis. The samples included sputum (n=145), tissue biopsy samples (n=25), cerebrospinal fluid (n=15), blood (n=14), pleural fluid (n=9), feces, (n=7), fluid from fistulae (n=2), and pus from a wound (n=1). The results obtained by PCR were consistent with those obtained with culture, which is the "gold standard." We demonstrate that PCR is a useful technique for the rapid diagnosis of tuberculosis at various sites

Results from the test bench of the Geometry Monitoring System of the ALICE Muon Spectrometer

We present the results obtained with the test bench of the Geometry Monitoring System (GMS) for the ALICE Muon Spectrometer. It consists in a mock up, reproducing at full scale, three half planes of the chambers 6, 7 and 8 of the spectrometer. We show that the GMS is able to measure transverse displacements with an accuracy of 1.5 microm. We show also that the resolution deteriorates by a factor 3 to 4 when thermal gradients are generated

Does subarachnoid haemorrhage affect the innate immune response?

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Normothermic mouse functional MRI of acute focal thermostimulation for probing nociception

Combining mouse genomics and functional magnetic resonance imaging (fMRI) provides a promising tool to unravel the molecular mechanisms of chronic pain. Probing murine nociception via the blood oxygenation level-dependent (BOLD) effect is still challenging due to methodological constraints. Here we report on the reproducible application of acute noxious heat stimuli to examine the feasibility and limitations of functional brain mapping for central pain processing in mice. Recent technical and procedural advances were applied for enhanced BOLD signal detection and a tight control of physiological parameters. The latter includes the development of a novel mouse cradle designed to maintain whole-body normothermia in anesthetized mice during fMRI in a way that reflects the thermal status of awake, resting mice. Applying mild noxious heat stimuli to wildtype mice resulted in highly significant BOLD patterns in anatomical brain structures forming the pain matrix, which comprise temporal signal intensity changes of up to 6% magnitude. We also observed sub-threshold correlation patterns in large areas of the brain, as well as alterations in mean arterial blood pressure (MABP) in response to the applied stimulus